Genomics and Transcriptomics
OPAL offers a comprehensive suite of leading-edge genomics and transcriptomics services powered by short- and long-read sequencing technologies.
We support end-to-end sequencing workflows, from DNA and RNA extraction and purification to library preparation, sequencing and bioinformatics.
We are proud to support projects of all sizes from amplicons to whole genome sequencing, as well as transcriptome analyses yielding insights into the identity and abundance of RNA transcripts from a range of sources.
Our genomics and transcriptomics platforms include instruments from PacBio (Revio, Sequel IIe), Illumina (NovaSeq 6000, MiSeq) and Oxford Nanopore Technologies (PromethION 24).
Applications
- Whole genome sequencing (WGS)
- RNA sequencing (RNA-Seq)
- Genotyping (low-pass WGS/skim-seq and targeted SNP sequencing)
- PacBio Kinnex and Iso-Seq
- Shotgun metagenomics
- Metabarcoding (16S, 18S, ITS)
- Hi-C library preparation and sequencing
- Amplicon sequencing
- ChIP-Seq
- Single-cell sequencing
Instruments
GIFS is committed to maintaining the highest operational standards. Sequencing specifications and performance can vary from that of the original equipment manufacturer. For user guidelines and recommended applications to support optimal results, Contact Us.
The Illumina NovaSeq 6000 provides short-read sequencing, generating up to 6 Tb or 20 billion reads in 48 hours with dual flow cell load.
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The Illumina MiSeq is a benchtop sequencer. With a 15 GB output per run and a maximum library size of 600 bp it’s ideal for small projects, including small genomes, target sequencing, amplicon sequencing and more.
The PromethION is ideal for large-scale projects. Up to 24 high-capacity flow cells can be run simultaneously, producing sequencing data from short to long (e.g. ≥ 15 KB) to ultralong (e.g. ≥ 4 MB).
HiFi reads from the Revio provide long-range information with high-base accuracy, resulting in data for high-quality plant, animal and microbial genome assemblies through a single cost-effective technology.
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